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This study set out to validate the use of 31 P-NMR spectroscopy together with alanine±glucagon infusions to assess hepatic gluconeogenic flux in vivo. Bolus infusions of alanine (2.8 or 5.6 mmol/kg)±glucagon (250 μg/kg) were used. Maximal changes in the phosphomonoesters (PME), inorganic phosphate (P i ) and β-NTP occurred 40 mins post infusion. PME increased 13.1% (p<0.02)...
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