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The binding of iron (Fe) to human serum transferrin (Tf) was analyzed with an HPLC system equipped with an anion exchange column and directly connected with a high-resolution inductively coupled plasma mass spectrometer for metal detection. The 56 Fe level in the eluate was monitored at resolution m/Δm=3000. Two monoferric Tfs were assigned based on the results of urea-PAGE and desferrioxamine...
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