To constrain the early Paleozoic tectonic evolution of the Jiamusi-Khanka Block and its relationship to the Late Pan-African event in Gondwana, we undertook zircon U-Pb dating and geochemical analyses (major and trace elements, and Hf isotopic compositions) of early Paleozoic intrusive rocks in the Jiamusi-Khanka Block, NE China. LA-ICP-MS zircon U-Pb age data demonstrate that these intrusive rocks were emplaced at three stages during the Late Pan-African event, represented by ~540Ma syenogranite, ~515Ma quartz syenite, and ~500Ma monzogranite and gabbro. Geochemically, the ~500Ma gabbros in the Jiamusi-Khanka Block have low SiO 2 (50.26-51.21wt.%), relatively high MgO (4.08-5.67wt.%), Ni (13.1-14.1ppm) and Cr (28.4-56.0ppm), and are slightly enriched in LILEs (e.g., Ba, K) and LREEs, and depleted in Zr, Hf, Nb, Ta and P. The ε H f (t) values of zircons in the gabbro range from +2.6 to +6.4. All these geochemical features indicate that the gabbros were likely produced by the partial melting of a depleted mantle that had been metasomatized by fluids derived from a subducted slab. In contrast, the ca.540-500Ma granites and quartz syenites contain high SiO 2 (64.49-72.20wt.%) and low MgO (0.40-0.75wt.%), Cr (1.69-6.88ppm) and Ni (1.26-3.26ppm). They have relatively low 1 7 6 Hf/ 1 7 7 Hf ratios of 0.282247-0.282599 with Hf two-stage model ages of 1173-2280Ma, and most of the magmatic zircons have positive ε H f (t) values varying from +0.2-+4.8, indicating that these granites and quartz syenites were probably derived from a dominantly Paleo-Mesoproterozoic ''old'' crustal source with possible different degrees of addition of juvenile materials. According to the geochemical data and global geological investigations, we propose that the 541-498Ma intrusive rocks in the Jiamusi-Khanka Block formed in a post-collisional or post-orogenic extensional setting linked to the collapse of a Late Pan-African orogen associated within the Gondwana.