The effects of ankle bracing on the levels and patterns of ankle muscle activation are not well understood.The purpose of this study was to examine this issue during a dynamic functional activity that involved marked ankle inversion.Within subjects repeated measures.University Research Laboratory.Fifteen female collegiate athletes completed multiple trials of a lateral shuffling movement, both with and without an ankle brace.Frontal plane kinematics of the ankle and EMG data of the peroneus longus (PL) were recorded during lateral shuffling test trials at four time points: prior to brace application, after brace application, following 2 weeks of continued use of the brace, and 2 weeks after discontinued use of the brace.The results demonstrated no significant changes in PL activity across the four time points.In contrast to previous reports, the use of an ankle brace does not produce immediate changes in muscle activation or changes in activation following short-term use.