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Recently, several papers regarding genes essential for somatic embryogenesis have been published. The most important genes playing a key role in both zygotic and somatic embryogenesis are: SERK, LEC, and BBM. The majority of them are regulatory genes coding transcriptional factors. It was proved that the highest transcript accumulation of AGL15, BBM, SERK genes is characteristic for early stages of...
Picea omorika (Pancic.) Purk. is an endemic species, threatened with extinction in the wild. Besides, it is a valuable species for nursery production. Hence it is necessary to protect its genetic resources ex situ. Somatic embryogenesis is a very beneficial micropropagation method because of potentially high regeneration rate and possibilities to maintain embryogenic tissue and somatic embryos in...
We examined the toxicity of two antibiotics belonging to the betalactam group, to carrot (Daucus carota L.) protoplasts. Leaf protoplasts were cultured in the presence of cefotaxime or carbenicillin applied in five concentrations in the range from 0.1 to 0.5 mg ml-1. Cell viability, division frequency, and regeneration capacity were assessed to determine the potential toxic effect of the antibiotics...
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