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A reversed phasc high performance liquid chromatography method (HPLC-RP) to quantify piroxicam was worked out. Piroxicam and naproxen -an internal standard, were extracted from human serum using soIid phase extraction (SPE) C(18) column. After eIution by methanol, the extracts were injected onto silica gel column (RP -select B) for HPLC with a U V detector set up a! 246 nm. The caIibration curve was...
An RP-HPLC assay to quantify hydrocortione in human serum after prior methylene chloride extraction has been developed for use in pharmacokinetic and/or bioavailability studies. The assay is simple, precise, accurate and sufficiently sensitive Methylprednisolone acetate has been proposed as a stable internal standard to increase the reproducibility of the assay. Mean extraction and processing recoveries...
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