The purpose of this study was to compare and quantify the angle difference among marker attachment methods for kinematic evaluation. For static evaluation, a hand mock-up was designed and used in single trials of different marker attachment methods. The mean absolute angle difference between the marker attachment methods and hand mock-up was not statistically significant. For dynamic evaluation, the gripping task began when a participant gripped a cylinder. The main effect of the marker set (p < .049) was significant. Thus, the use of one marker per joint is recommended for static evaluation because it causes less discomfort when a patient moves his/her hand and because utilizing the same marker placements for each subject is easy. For dynamic evaluation, the use of three markers per segment or a cluster marker is recommended because they are less affected by skin movement.