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A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification. The specificity of the method was validated by single PCR tests performed with...
Eighty isolates of Escherichia coli were collected in Northern Palestine throughout the 1996 to 2000 period from hospitalized patients with urinary tract infections (UTIs). Resistance rates were ampicillin, 65%; co-trimoxazole, 55%; cefuroxime, 10%; cefotaxime, 7.5%; ceftazidime, 2.5%; ciprofloxacin, 12.5%; gentamicin, 6.25% and amikacin, 1.25%. No imipenem-resistant isolates were identified. To determine...
Pseudomonas fluorescens strains III107 and II21 and Bacillus mycoides strains JC192 and K184, stimulating growth of winter wheat, were chosen for the studies. The bacterial strains inhibited on agar nutrient medium the growth of Gaeumannomyces graminis var. tritici (Ggt) - the pathogenic fungus causing take-all on wheat. Both strains of pseudomonads synthesized relatively high amounts of Fe³⁺ chelators...
In 48 adult women, subdivided into group 1 with no cervical intraepithelial neoplasia (CIN-negative) and group 2 (CIN-positive), endocervical scrapes were tested for the presence EBV DNA and HPV DNA using PCR-ELISA. In addition, attempts were made to detect HPV 16 and HPV 18 using other PCR amplification techniques. In parallel, in biopsies of uterine cervix obtained from group 2 patients, presence...
In this study, favorable carbon-nitrogen ratio for high yields of gibberellic acid (GA₃) production from Pseudomonas sp. was investigated. First of all, optimum carbon (glucose, maltose, sucrose, fructose, lactose) and nitrogen (KNO₃, NH₄C1, NaNO₃, urea, glycine) sources among the others were chosen. The highest yield of GA₃ productivity was found in growth medium supplemented with fructose (168.5...
The content of fatty acids extracted from the membranes of E. coli MC1061 harboring the wild-type dnaKdnaJ alleles and its AdnaJ and AdnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42°C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and octadecanol, the latter...
Resistance to antituberculous agents is an important cause of ineffectiveness of antimicrobial therapy. The resistance of M. tuberculosis to antituberculous agents is a result of mutations in genes participating in those agent's action. The antituberculous drug - isoniazid can be activated by Mycobacterium tuberculosis either through a hydroperoxidase I/II or a superoxide-dependent oxyferrous pathway...
Listeria monocytogenes is an opportunistic pathogen that causes infections collectively termed listeriosis, which are related to the ingestion of food contaminated with these gram-positive rods. The pathogenicity of L. monocytogenes is detennined by the following virulence factors: listeriolysin O, protein ActA, two phospholipases C, internalins (InlA and InlB), protein CwhA and a metalloprotease...
The phytohormone IAA (indol-3-acetic acid) was tested in vitro on growth of tomato wilt pathogen Fusarium oxysporum lycopersici. The hormone reduced spore germination, mycelial dry weight and protein content. Such reduction was matched with the elevation in the hormone concentration. The in vivo application of IAA to soil of the uninoculated plants (controls) improved growth and yielded longer shoot...
In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early - pUL44(DB52) and late - ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flow cytometry in human leucocytes, isolated, infected and cultivated in vitro was...
Shiga-like toxin-producing (SLTEC) Escherichia coli strains are one of the most important food borne emerging pathogens. One hundred and fifty-seven E. coli strains isolated from 39 children with diarrhea of unknown origin and one hundred and five E. coli strains from 20 healthy children were examined for Shiga-like toxin production in Vero cell line assay. The synthesis of Shiga-like toxin was observed...
Inoculation of wheat seeds with two strains of Pseudomonas fluorescens (III107 and II21) and two strains of Bacillus mycoides (JC192 and K184) isolated from winter wheat roots, as well as with one strain of P. fluorescens (ID13) isolated from oat roots, reduced the negative influence of Fusarium culmorum on winter wheat in a 28 day pot experiment. The bacterial strains (especially III107 and chitinolytic...
The interaction of CD28 with one of the B7 molecules (CD80 and CD86) on professional antigen-presenting cells (APC) is generally considered to be the most important co-stimulatory signal for T cell activation. Several lines of evidence suggest that dendritic cells (DC), the most potent antigen presenting cells known, play a role in the immunological control of herpes simplex virus (HSV) infections...
Clostridium difficile has become the most common cause of hospital acquired diarrhea after antibiotic treatment. The aim of this study was to determine the frequency of C. difficile associated diarrhea among hematology/oncology ward patients and to characterize isolated strains. Twenty three toxigenic and thirteen non-toxigenic strains were detected among fecal isolates. Antibiotic susceptibility...
Transposable elements (i.e. insertion sequences and transposons) are components of nearly all bacterial genomes. The majority of these elements have been identified as a result of various sequencing projects. However, in most cases, their activity was not experimentally confirmed. For this reason several strategies have been developed that allow direct cloning and identification of functional transposable...
The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, from a Haematological Unit patient in the Clinical Hospital in Gdańsk. PCR-RFLP analysis...
In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in Egypt...
The studies focused on the effect of the addition of low concentrations of glucose - a substrate that is readily subject to microbiological degradation, on the rate of biodégradation of petroleum products. Glucose in concentration 1% was introduced into mineral medium containing 3% crude oil, with or without bacterial inoculum. The rate of degradation of crude oil in the individual cultures was determined...
Studies were conducted on the enhancement of pathogenicity of Bacillus thuringiensis by gamma rays on various species of termites. The results showed that the 72 hrs old and 60 or 70 kr irradiated culture of B. thuringiensis were more pathogenic than the non-irradiated ones. 168 hrs old and 150 kr (1.5 kGy) irradiated B. thuringiensis caused quicker mortality rate than the non-irradiated one as it...
The association between the presence of self-splicing intron Ca.LSU and proteolytic activity of Candida albicans isolates was tested. Study included 95 C. albicans strains isolated from gastrointestinal tract of diabetes children. The strains with the intron (genotype B) displayed a significantly higher proteolytic activity (385.2 ± 192U/L) than did strains without intron (genotype A) (119 ± 115U/L)...
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