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Nematodes from the superfamily Ascaridoidea (families Anisakidae and Raphidascarididae) are worldwide distributed parasites. Their live cycles include many species of water invertebrates and teleostean fish as intermediate hosts, and fish, sea mammals or fish-eating birds being definitive hosts. Humans can be infected with some of these parasites after consumption of raw or wrongly processed fish...
Mutants of Mesorhizobium ciceri BICC 651 were generated by N-methyl-N'-nitro-Af-nitrosoguanidine mutagenesis. Siderophore overproducing mutants were identified on Chrome azurol S agar plates. One of them determined as N15 was examined for symbiotic efficiency and compared to its wild type parent i.e. BICC 651 strain under sterile conditions using Leonard jars in growth chamber and also in pots containing...
Opportunistic infections are usually caused by endogenic flora originated from physiological flora. In this context we studied coryneform bacteria recovered from deeper layers of epidermis of the forehead (278 isolates) and the back (196 isolates) of healthy men. It was observed that coryneform bacteria are in dynamic equilibrium with coagu-lase-negative staphylococci and they amount 4,7% of resident...
Ethambutol (EMB), the first line drug in the treatment of tuberculosis, is an inhibitor of the biosynthesis of the cell wall compound - arabinogalactan. It was found that EMB at sub-inhibitory concentration increases the permeability of the M. vaccae cell wall, which was monitored by cell sensitization to erythromycin and rifampicin. The high permeability of the cell wall to hydrophobic compounds...
Alternaría alternata were isolated and identified from root, foliage and soil of both wheat and rice crops and their aggressiveness was studied using aggressiveness analysis. Isolates of Alternaría alternata were genetically characterized using RAPD's. The investigations were based on surveys of wheat and rice crops in the rice-wheat cropping areas of Pakistan. The study showed that Alternaría alternata...
The laboratory experiments tested the influence of selected pesticides on the symbiotic efficiency and nitrogenase activity of Rhizobium teguminosarum bv. trifolii KGL, Sinorhizobium melilotii Bp and Bradyrhizobium sp. Ornithopus B bacteria entering into symbiosis with clover, lucerne and serradella, respectively. The results obtained indicate that the pesticides used in the experiments (Funaben T...
The efficacy of five disinfecting agents was tested by a new microbiological method, i.e. the flow cytometry. The method is based on kinetic measurements of the effects of a disinfecting agent on the percent of live/dead cells detected with propidium iodide. E. coli ATCC52922 strain and S. aureus ATCC 29213 strain were used in the experiment. From the measurements the killing time 50 (KT50) was estimated...
Coagulase-negative staphylococci (CoNS) have become the most often isolated bacteria from blood culture, spinal fluid and respiratory tracts of neonates. These nosocomial strains are often resistant to oxacillin and other antibiotics (macrolides, aminoglycosides and fluorochinolones). 50 multidrug resistant CoNS strains isolated from bloodstream neonatal infections were tested for sensitivity to 23...
A natural rubber degrading candidate was isolated from a soil sample from Aswan, Egypt. The strain was able to grow on natural rubber as a sole source for carbon and energy. According to its degradation behavior, it grew adhesively and in direct contact with the rubber substrate and led to disintegration of the material during cultivation. Furthermore, this strain was not able to form a clear zone...
Activity of the enzymes can give information on the kind and duration of the effects of pollutants on the metabolic activity of soil. Results of studies on the dehydrogenase activity in soils experimentally contaminated with diesel fuel, with and without modification used in investigation over biodégradation process (including fertilization, stirring and inoculation) were presented in paper. Studies...
Enterococcal infection has become a major clinical problem and E. faecalis and E. faecium are the most frequently isolated species. However, the isolation of other species (E. casseliflavus, E. gallinarwn, E. durans) from clinical materials was reported recently. The aim of this study was to evaluate drug resistance of 97 species of enterococci isolated from clinical specimens of Upper Silesian Health...
During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of...
The aim of this study was to evaluate the reduction in the adherence of 33 strains of Pseudomonas aeruginosa isolated from humans and different animals to human buccal epithelial cells with neuraminidase inhibition. Buccal epithelial cells were incubated with strains of Pseudomonas aeruginosa in the presence or absence of the neuraminidase inhibitors, 2,3-dehydro-2-deoxy-N-acetyl-neuraminic acid (DANA)...
A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasms proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our results have...
In Staphylococcus aureus B47 grown in iron-restricted medium, six new, iron-regulated proteins occurred in cytoplasmic membrane. Protein of 14kDa has bound two complexes of iron: Fe(III)-staphylobactin and Fe(III)-acinetoferrin. Complexes of Fe(III)-ferrichrome and Fe(III)-rhodotorulic acid were not bound to any of new membrane protein. Iron of Fe(III)-staphylobactin and Fe(III)-acinetoferrin complexes...
Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP In the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdansk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated...
The study has been carried out to determine the frequency of C. difficile recovery in stool cultures and the rate of C. difficile toxin A detection in faecal specimens of patients with nosocomial diarrhoea. Clinical specimens comprised 4414 stool samples collected from 1998 to 2002 from adult patients hospitalised in different wards of a university-affiliated hospital (1200 beds) and suspected of...
The collection of 314 staphylococcal strains including Staphylococcus aureus and coagulase-negative staphylococci (CNS) was isolated from skin or nasopharynx of healthy people. It was found that the majority of staphylococci possessed the ability to produce slime intensively or moderately, irrespective of ecological niche-nose, throat or skin. Most of them showed the hydrophilic cell surface. However,...
Two bacterial strains identified as Ralstonia picketti (BP 20) and Alcaligenes piechaudii (CZOR L-1B) were isolated from petroleum hydrocarbon-contaminated soil following bioremediation treatment. The surface active properties, e.g. surface tension, emulsification and foamability of their culture filtrates were evaluated. Bacterial cell-surface hydro-phobicity (BAH) as measured by analyzing cell affinity...
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