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The incidence of extended-spectrum β-lactamases (ESBLs) was analyzed in Enterobacteriaceae population circulating in the Upper Silesian Child and Mother Health Center in Katowice (USC&MHC). Altogether 1164 clinical specimens, collected from children hospitalized in 8 different hospital units of USC&MHC were investigated. Five hundred and eighty-five clinical isolates of the family Enterobacteriaceae...
Although integrons by themselves are not mobile, due to their presence in plasmids and transposons, they can be transferred horizontally. For these reasons integrons are a major mechanism for the spread and maintenance of multidrug resistance (MDR). This study describes the distribution of integron gene cassette classes in a collection of uropathogenic Escherichia coli (UPEC) isolated from children...
Gut microbiota was analyzed in children, aged 6–18 months and su$ering from atopic dermatitis before and after 3 month supplementation of their diet with Lactobacillus casei DN – 114001 in a dose of 10⁹ cells daily. On completion of this period the total number of fecal Lactobacillus sp. cells decreased from 7.86 Log₁₀ CFU/g to 6.40 Log₁₀ CFU/g. After the next 5 months (without dietary supplementation...
The aim of the study was to analyze the differences in the activity of β-glucuronidase and β-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation...
There is a 10–30% prevalence of HP infection in the general pediatric population in Poland. This study aimed to determine its prevalence in T1DM children in Upper Silesia, Poland and estimate its influence on metabolic control of patients. We studied 149(82♀) children with T1DM (duration > 12 months, mean HbA1c) and 298(164♀) age-matched controls. In all cases height and weight z-scores and Cole’s...
The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic...
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