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The performed experiments allowed investigating a strain of Candida bombicola ATCC 22214 yeasts from the point of view of its potential to manufacture extracellular lipase and its activity in relation to the applied fat substrate. Oleic acid, sunflower oil and a post-deodorizing condensate were used as culturing substrates. It was found that the best agent stimulating yeast enzymatic system to produce...
The investigation included evaluation of milk obtained from 97 hf cows imported from France and 119 bw cows of local population with different share of hf breed. Milk from cows of the two populations was characterized by the proper freshness (pH value, potential acidity) meeting the criteria for purchase (PN-A-86002:1999), and a high hygienic quality (TNM, SCC) allowed them to be qualified to the...
Rhizopus cohnii lipase was immobilized by adsorption on porous chitosan polyphosphate beads. The obtained preparation, active against fatty acids esters of p-nitrophenol and against olive oil, was characterised and compared with the free enzyme. Both lipase forms displayed the highest activity at pH 8-9. The optimum activity temperature of free and immobilized lipase was 37°C and 30°C, respectively...
Healthy diet is one of the most important factors determining somatic and mental development of children. The reported study aimed at assessing food intake and nutritional status of 600 children aged 11-14, pupils of primary schools in the city of Poznań. The 24-h recall was taken to evaluate the total intake of basic nutrients, i.e. proteins, fats and carbohydrates. The nutritional status of children...
The effect of three mixtures of hydrocolloids containing 1.5% protein isolate from cattle blood plasma AMP 600N and 0.2% carrageen E407, 1.5% protein isolate from cattle blood plasma AMP 600N and 0.4% carrageen E407, 1.5% protein isolate from cattle blood plasma AMP 600N and 0.4% satalginate S550, on the functional properties, the amount of frozen water and lipid oxidation in minced pork, stored at...
Of polycyclic aromatic hydrocarbons (PAHs) present in food, benzo(a)pyrene (B(a)P) is the most often determined compound as a surrogate for all PAHs. Various chromatographic procedures, including gas, liquid and thin layer chromatography have been applied to isolate these substances from contaminated samples. Because of the complexity of food matrices additional clean up of extract prior chromatographic...
Three groups of crossbreeds of wbp x pbz breeds (10 fattening pigs in each group) were fed Starter (30-70 kg) and Finisher (71-105 kg of body weight) all-mash which contained triticale meal, barley and different kinds of protein feeds. Fattening pigs of the control group (K) were given mixtures with extracted soybean meal, W groups - mixtures with rapeseed cake and fodder pea. Meat-bone meal was introduced...
The effect of temperature during hydrogénation of soybean oil on the degree of poisoning of nickel catalyst by phospholipids was investigated. Two wide porous catalysts with diverse particle sizes were used: catalyst No. 1, medium-grained and catalyst No. 2, fine-grained. Hydrogénation was conducted in a "dead end" type laboratory scale reactor in the temperature range of 160÷190°C. The...
Changes in the volume of cylindrical (40 mm in diameter, 2.5, 5 and 10 mm in thickness) samples of apple variety Lobo were determined after drying with infrared radiation whose intensity corresponded to power consumption by radiators equal to 200, 550, 900 and 1250 W. These measurements provided the basis for determining a correlation between shrinkage, infrared radiation and the thickness of the...
The aim of the present study was to evaluate the effects of enriching the uncorrected diet of post-mastectomy women with a hull-less oat Akt cultivar on the subjects' blood glucose, triacylglycerol, total cholesterol, HDL- and LDL-cholesterol, fibrinogen, and estradiol levels. The cholesterols were assayed in a closed system, with Roche reagents in an Integra biochemical analyser. The fibrinogen level...
The aim of the work was to determine the effect of sterilization of pork in the presence of oxidized lard and selected antioxidants on quantitative and qualitative changes of thiamine in pork. The antioxidants were casein hydrolysate and rosemary extract. It was found that sterilization itself caused the greatest losses in both forms of thiamine. In the case of adding fresh lard to the meat, the total...
Synthesis of extracellular lipases by Candida antarctica was induced by adding fat or oil to the culture medium. The highest lipase activity (9.1 U) was obtained after 72 h of cultivation of yeast in medium supplemented with 2% (v/v) l,2,3-trioleoyl-sn-glycerol. On the basis of SDS-PAGE analysis, Candida antarctica synthesized two forms of lipase having molecular weight of 36 and 52 kDa. Lipase from...
The oxidative modification of pea and bean proteins caused by hydroxyl radicals was investigated. The changes that occurred during the oxidation process were examined using fluorescence detection and electrophoretic methods. The modification of proteins resulted in a loss of Trp residues and a gain in its degradation product - formylkynurenine (FK); Trp residues were oxidized to bityrosine (BT). The...
Germination (in light and dark, at 25°C, for up to 7 days) of cruciferous seeds was studied to determine the optimum conditions to maximize the synthesis of vitamin E in sprouts. In this study, the tocopherol content (α-T, ß-Т, y-T, δ-T) was examined in four species of cruciferous seeds: rapeseed (Brassica napus var. oleifera), white mustard (oleiferaSinapis alba L.), radish (Raphanus sativus L.),...
Whey proteins and polysaccharides are two gelling biopolymers widely used in the food industry. Gelation of whey protein isolate (WPI), WPI-k-carrageenan and WPI-λ-carrageenan mixed systems has been investigated. The apparent viscosity of WPI (3%) solutions significantly increased after polysaccharide addition. The highest apparent viscosity was observed at pH 7 and 0.3% к-carrageenan. The texture...
Measurement of colour is one of the methods for evaluating shortcomings in the quality of meat type PSE or DFD. There are no uniformly specified extreme values L* for the evaluation of faulty meat of gallinaceous poultry in the scientific literature. A uniform procedure for this measurement is also non-existent. The aim of this experiment was to determine the effect of colour measurements done in...
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