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Beta-casein gene (CSN2) is considered a marker of milk production traits in dairy cattle. Allele A2, the most common in Holstein-Friesian breed, is beneficial for milk protein yield. However, within CSN2 other mutations occur which could potentially be involved in shaping the variation of milk production traits. In this study a new SNP (C/T) was found located within a regulatory sequence of CSN2 (position...
CXC chemokine receptors 1 (CXCR1) play a key role in inflammatory response and eventual activation of the innate immunity. Mastitis is a big problem in dairy industry. In the presented study the bovine CXCR1 gene was considered a candidate gene of anti-mastitis defense in Chinese Holsteins.Polymorphism of bovine CXCR1 was investigated and its associations with Somatic Cell Scores (SCS) and milk production...
The insulin-like growth factor (IGF) signalling pathway plays a crucial role in the regulation of growth and development of mammals. The strong mammary insulin-like growth factor receptor type 1 (IGF1R) mRNA expression during lactation suggests a role for peripheral insulin-like growth factor I (IGF-I) in maintenance of lactation. The aim of the study was to identify the frequencies of alleles and...
The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBB gene, two pairs of primers were designed to detect single nucleotide polymorphism in exon 2 of INHBB gene in high-prolificacy (Jining Grey)and low-prolificacy (Inner Mongolia Cashmere and Angora) goats by polymerase chain reactionsingle strand conformation...
Lipid profile of intramuscular fat (IMF) from semitendinosus muscle of lambs was examined in relation to the intensity of fattening system, animals’ final body weight and sex. Conducted were two repeations of fattening (2 x 32 lambs) each based on intensive (IN, 16 lambs) vs. semi-intensive (SIN, 16 lambs) feeding system, continued up to lower vs. higher slaughter weight, and with reference to sex...
Used were 8 castrated males and 8 entire females of indigenous Casertana and Large White pigs, 4 animals of each breed. In Longissimus dorsi (LD) and Psoas major (PM) muscles the morphometric characteristics of FG (fast glycolytic), FOG (fast oxidative glycolytic) and SO (slow oxidative) fibre types was performed and their percentage determined. Determined was also the area of adipocytes from internal...
The aim of the study was to evaluate the effect of the dietary linseed and rapeseed supplements on the physico-chemical and sensory properties of ostrich meat. The experiment was carried out on forty growing ostriches raised in five groups (control and with 4 and 8% linseed and 5 and 10% rapeseed of the diet). The diet with rapeseed did not affect the physico-chemical properties of meat,while 4% linseed...
The physiological efficiency and mechanisms of action were studied of gonadotropin releasing hormone analogue (GnRH-A) on the reproductive functions of rabbits. Twenty-four female rabbits (Oryctolagus cuniculus) were randomly divided into four groups. Group EG-1, EG-2 and EG-3 were subcutaneously injected with 1.0 mL GnRH-A antigen at 100 μg/mL, 100 μg/mL and 50 μg/mL. EG-2 and EG-3 were re-injected...
The beta 3-adrenergic receptor (ADRB3) is a G-protein coupled receptor encoded by ADRB3 gene and involved in regulation of lipolysis and homeostasis. The objective of this study was to detect the genetic variation existed in this gene in eight domestic and wild pig populations. The polymorphism of partial sequences of ADRB3 gene in 336 pigs was analysed by single strand conformation polymorphism technique...
The study utilized age-dependent breed-specific gene expression profiling by cDNA-AFLP technique in identification of differentially expressed (DE) candidate genes for growth and development trait. Results revealed 15579 transcript derived fragments (TDFs) expressed in bovine pituitary gland tissue using 96 unique PCR reactions with primer combinations (PC) of TaqI-MseI for Polish Holstein (PH) and...
The study aimed at analysing and comparing haematological indices, blood saturation and dimensions of red blood cells of Ross 308 broiler chickens of both sexes. On day 44 of rearing, blond was withdrawn once from 60 randomly selected chickens (1:1 sex ratio). Prior to blood withdrawal the chickens were weighed and their heart rate and haemoglobin saturation were assessed. The following blood parameters...
The aim of the study was to describe polymorphism of BoLA-DRB3.2 in Polish Holstein-Friesians. Alleles were identified according to PCR-RFLP procedure using a modification to the van Eijk’s proposal [1992]. Restriction patterns were obtained with BstYI, HaeIII and RsaI enzymes. The study presented variability of all the alleles estimated in two herds. 752 animals were genotyped and informative according...
As a member of tyrosinase-related family, tyrosinase-related protein-1 (TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about its role in goat. In this study, almost complete goat TYRP1 gene (17554 bp, HMO70243) encompassing complete CDS was identified, the coding region amounting to 1614 bp, distributed in 7 exons (2-8), and...
The first description is presented of nucleotide sequence of exon 2 of caprine melatonin receptor 1b (MT2). No polymorphisms of MT2 gene were detected between high fertility and year-round estrous goat breeds and low fertility and seasonal estrous goat breeds. It is likely that exon 2 of MT2 gene is not associated with fertility or reproductive seasonality in goat breeds. The nucleotide sequence of...
The study suggests that Sirtuin 1 (SIRT1) may play an important role in the development of bovine adipose tissue in vivo. To investigate the differential mRNA expression between bovine intramuscular (IM) preadipocytes (BIPs) and subcutaneous (SC) preadipocytes (BSPs), conventional collagenasebased procedures were modified to harvest preadipocytes from the longissimus dorsi muscle and SC adipose tissue...
The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein...
The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this polymorphism was excluded from the assay. Such genotyping problems were not observed in earlier studies describing the method and cannot be easily explained. However, full accordance...
The aims of the current study were to evaluate the effects of diazepam (D) and/or xylazine (X) on the induction of sedation and anesthesia, performing ovarian transplantation and litter size upon administration during pregnancy. One hundred and five albino rat females were classified randomly into four groups given intraperitoneal (IP) injection of physiological saline (group I), diazepam (6.2 mg...
Experimental flock, a total of 105 birds, was divided into 5 groups (with three replications), 15 females and 6 males in each. Group I (control) was fed a complete feed mix of standard composition. Feed mix for groups II and III was the control one with 4 and 7% common flax seeds (CFS), whereas that for groups IV and V was the control one with 2 and 5% black cumin seeds (BCS) added, respectively....
The study aimed at cloning the complete cDNA sequence of porcine CDH1 gene and detecting its expression in different developmental stages of early parthenotes. After cloning the CDH1 gene from porcine oviduct using rapid amplification of cDNA ends (RACE) method, the sequence analysis revealed that porcine CDH1 gene complete cDNA nucleotide sequence amounted to 4283 bp including 2652 bp of open reading...
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