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In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use...
The aim of this studies was the genetic analysis of Polish SVDV isolates from 1972-73. The direct nucleotide sequencing technique of PCR products from the 1D (VP1) structural polipeptyde coding region was applied. The genetic differences between Polish isolates and other European and Japanese ones from 1966-1994 were determined by comparing their nucleotide sequences. No considerable genetic divergences...
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