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In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use...
One hundred and seventy-six samples collected from internal organs of sick birds and 40 samples taken from healthy birds were used for virus isolation. The isolation was carried out on SPF chicken embryos by inoculating them into the yolk sac with the material prepared by generally accepted methods. Three passages were performed. The presence of viral antigen was detected by the agar gel precipitation...
The aim of this studies was the genetic analysis of Polish SVDV isolates from 1972-73. The direct nucleotide sequencing technique of PCR products from the 1D (VP1) structural polipeptyde coding region was applied. The genetic differences between Polish isolates and other European and Japanese ones from 1966-1994 were determined by comparing their nucleotide sequences. No considerable genetic divergences...
Studies on effects of pasteurisation of meat with spiked hormones at residue levels have been made. Beef and poultry meat samples which contained diethylstilbestrol and 19-nortestosterone were heated at 80°C for 20 minutes. The hormones were quantified by high-performance liquid chromatography with UV detection on raw and pasteurised meat samples. Diethylstilbestrol is stable under these conditions...
The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
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