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The stability of 2 H. bovis antigens prepared by different methods was examined by the ELISA. It was found that the activity of H. bovis secretory antigen kept at - 18°C and -70° C and full antigen stored at - 18°C was significantly decreased as early as 6 months after storage. Lyophilized, secretory H. bovis antigen showed constant, high activity and may be used for one year at least.
The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically...
Investigations aimed to evaluate the effectiveness of Boviseptivac, vaccine against bovine pasteurellosis prepared from the Polish isolates of serotype B:2 with an oil adjuvant (Emulsigen). The vaccine protective value obtained on white mice was 4.25 according to the method of Ose and Muenster. Field trials carried out on 120 cows in the Ostroleka province revealed advantageous properties of the vaccine,...
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