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The aim of the experiment was to determine, using the ELISA and gel diffusion precipitin test, the antibody serum level in rabbits immunised with vaccines comprising P.multocida antigens of serotypes 3 and 12 with two adjuvants: oil (Cunipastivac E) and aluminium hydroxide gel (Cunipastivac A). The ELISA revealed an increase in specific lgG levels against P. multocida and the positive precipitin reaction...
The experiment was performed on 24 ewes divided into 4 groups. Experimental sheep were injected parenterally either with a sodium selenite and Emulsigen mixture or sodium selenite or Emulsigen. The injection of sodium selenite and Emusigen mixture, and Emulsigen alone substantially increased serum copper and magnesium concentrations. Moreover, a marked decrease in creatine kinase activity was found...
Whole blood samples from 73 animals from a dairy herd infected with BLV were used for detection of proviral DNA by means of PCR technique. At the beginning 24 cows were examined with primer set ZM1/ZM2. Seven out of 13 ELISA-positive animals –were PCR-positive too and 3 were PCR-positive among 11 serologically negative. Due to unsatisfactory results the rest of the samples were tested with a second...
Results of nitrite and nitrate residue determination in cured meat products performer in veterinary laboratories and in the Department of Hygiene of Food of Animal Origin in the National Veterinary Research Institute in 1995 are presented. Measurements were conducted according to the Polish Standards. Mean nitrate and nitrite concentrations in meat products were 18.6 ± 14.8 to 49.7 ± 101.6 mg/kg and...
The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA...
A new ELISA method for VHDV diagnosis (indirect sandwich ELISA) and Iiquid-phase blocking ELISA (LPBE) for the determination of antibodies to VHDV were developed. These assays are sensitive and specific and results may be directly correlated to those recorded by HA or HI. Besides, the presented techniques are rapid and relatively simple to perform and reagents are used economically. Therefore, the...
The polymerase chain reaction method (PCR) has been applied to the detection of FMD viral RNA in samples taken from the calf during the clinical stage of FMD. Total RNA was extracted with a guanidinum thiocyanate-phenol-chloroform method and reverse transcribed using AMV-reverse transcriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence. The amplified...
The standard strain and 78 field samples of rabbit haemorrhagic disease were tested by the haemagglutination test (HA) and ELISA. The occurrence of 17% false negative results in HA test was demonstrated. The results of studies of 30 out of 78 samples examined by the ELISA prepared in our laboratory in comparison with commercial kit are presented. The high correlation of results obtained in ELISA kits...
The influence of ß-haemolytic Streptococcus organisms at concentrations of 100 x, 200 x, and 500 x 10³ cells/ml sperm incubated at 37°C for 60 min with stallion semen suspension on the motility, viability and morphology of the semen, and AspAT activity in seminal plasma was examined. S. zooepidemicus at a concentration of 500 x 10³ cells/ml exhibited a particularly unfavourable effect on semen. All...
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