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Occurrence of borreliosis in human population is associated with possibility of contact with the biological vector of this disease - a common European tick, Ixodes ricinus. Therefore, the highest number of cases of Lyme disease has been recorded among forestry workers and inhabitants of wooded areas. Diagnostics of borreliosis is based on immunoserologic tests - ELISA or indirect immunofluorescence...
The presence of antibodies against Chlamydophila (Chlamydia) psittaci in small mammals (Insectivora, Rodentia) in the region of East Slovakia are presented. The hosts were caught in several areas of Slovakia in habitats with different levels of anthropogenic disturbance. Research was carried out during 2000-2002. The authors examined 1,947 sera coming from 4 insectivore and 10 rodent species. Each...
The seroprevalence of human granulocytic anaplasmosis (former human granulocytic ehrlichiosis, HGE) has been documented in several studies, but little data exists on incidence rates in healthy individuals. In a prospective study, we tested 125 healthy adults (mean age 43 years) - workers of the Białowieża Primeval Forest National Park, north-eastern Poland - for Anaplasma phagocytophilum IgG antibodies...
Both the presence of Anaplasma phagocytophilum in ticks and the seroprevalence of human granulocytic anaplasmosis have been reported in different parts of Europe. There are few reports concerning this problem in Poland. The aim of the study was to assess the prevalence of Anaplasma phagocytophilum in ticks, and to detect antibodies against the HGE agent in serum of forest workers in the region of...
The study was undertaken to investigate the prevalence and clinical manifestations of Mansonella perstans infection in the nomadic Fulanis of northern Nigeria. Physical examination of 755 consenting nomads for clinical manifestations of M. perstans infection was carried out between June 1996-September 2000. This was followed by parasitological examination of blood samples collected by finger prick...
The prevalence of Francisella tularensis, Borrelia burgdorferi and Anaplasma phagocytophilum in rodents is a determinant for their role in maintaining pathogens in the environment. A total of 169 rodents, trapped in an endemic focus of tularemia, were examined by PCR to asses the frequency of infection with the etiological agents of tularemia, Lyme borreliosis and human granulocytic anaplasmosis....
During the period of 2000-2004, 3,096 red foxes from the whole territory of the Slovak Republic were sampled and examined parasitologically for infections with Echinococcus multilocularis, causative agent of serious alveolar echinococcosis in humans. Relations between prevalence of the parasite in individual regions of Slovakia and some environmental factors were weighted. During the study period,...
The aim of the study was to analyse the prevalence of H. pylori infection in adult inhabitants of Lublin Province. The effects of living conditions and lifestyle on the infection frequency were evaluated. The study included 585 adults randomly chosen for the epidemiological analysis of H. pylori infection in the Lublin region within the project commissioned by the Ministry of Health (PCZ 08-09) and...
A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected by fl agging of vegetation in suburban woods of Pisárky Park (city of Brno) from July to October 2002. The midgut of each tick was dissected out and transferred individually into BSK-H medium. After cultivation, all specimens were examined by dark-fi eld microscopy (DFM) for the presence of borreliae. Out of 305 tick...
In this study we report the usefulness of nested PCR for screening of the persistent B. microti infections in rodent hosts. Female BALB/c mice were inoculated with 100 μl of donor blood infected with B. microti. Infections were detected using microscopic examination of Giemsa-stained blood smears. To determine whether B. microti DNA was present in blood and/or spleen tissue, nested PCR was performed...
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