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Ten cows with and 5 cows without puerperal endometritis were treated with 20 μg GnRH analogue, buserelin, intramuscularly. Blood samples were taken every 20 min for 8h before and for 8h after injection of GnRH. All cows responded to GnRH with significant increase of LH 20 min after treatment. The LH peak was significantly lower in cows with puerperal endometritis than that in clinically normal cows...
The investigation aimed at preparing an ELISA kit for examination of bovine sera for brucellosis and evaluating the diagnostic properties of the test. Lipopolysaccharide extract (LPS) was used as the antigen for microplate coating, antibodies against IgG of bovine sera with horseradish peroxidase were used as the conjugate and ABTS with H202 as the substrate. A weak-positive serum prepared from the...
In a previous paper an ELISA for detecting anti-Brucella antibodies in cattle sera was elaborated and evaluated. The aim of this study was to evaluate the ELISA for testing individual milk samples from cattle. Validation was performed by comparing the results of this test with those in the Rose Bengal Test (RBT), tube agglutination test (TAT), complement fixation test (CFT), Coombs anti-globulin test...
The objective of this study was to examine the effects of 100, 300 and 500 MPa hydrostatic pressures on the morphology of muscle fibres and texture (tenderness and toughness) of the muscles Longissimus thoracis and Gluteus medius taken from chilled carcasses of Black-and-White cattle and Polish Large White pig. Histological examination, scanning electron microscopy (SEM), morphometric analysis (indices...
Lymphocytes of haematologically and serologically EBB-positive cows were isolated and incubated with CD4, CD8, Cdllc and alpha bovine IgM markers, produced by Int. ILRAD Peter Maesseus. The cells were analysed immediately following fluorescein marking, in flow cytometer (FACS Calibur - Becton Dickinson). The number of proliferating B lymphocytes with increased IgM marker expression was high, testifying...
The expression of metallothionein (MT) was investigated immunohistochemically in the cells of total blood smears of cows infected with bovine leukemia virus, smears from in vitro culture of1 leukemic lymphocyte and in histological preparations of leukemic affected lymph nodes. In the blood smears cells and in -vitrtréulture lymphocytes, MT, was localized under cellular membrane in the form of fine...
A cannula was inserted into the aorta abdominalis through the coccygeal artery (cranial to the origin of the ovarian artery) in mature heifers (n=4) and in old cows (n=4) with reproductive disorders, to facilitate infusion of noradrenaline (NA; 4 mg/30 min) on day 10 of the oestrous cycle. Before, during and after NA jnfusion peripheral blood samples were collected for progesterone and oxytocin determination...
Technological advances in last decades of XX centuries were well utilised in the studies of biological oxidation processes. Biological oxidation can lead to the oxidative stress and subsequently to the cell damage of animal organisms leading to many diseases. Free radical processes taking place in cattle under pathological conditions. Metal ions are often responsible for the damage of biological systems...
The aim of this study was to evaluate the occurrence of antibodies to foot-and-mouth disease virus (FMDV) in sera of cattle in Poland. The examinations were performed using the virus neutralization (VN) test and ELISA methods: liquid-phase blocking ELISA (LPBE) and 3ABC-ELISA. During 1993-2001, about 681 000 samples of sera collected from animals held on the territory of Poland were tested. Of about...
In this article we have presented genetic predispositions and the influence of numerous factors that occur during cattle fattening, transport and holding in slaughter plants, on their welfare and subsequently on the occurrence of defective meat, known in the literature as DFD (Dark, Firm, Dry) meat. Such meat is unwillingly bought by contemporary consumers because of its consistency and processing...
Mannheimia haemolytica leukotoxin (Lkt) is the major factor that contributes to lung injury in bovine pneumonic pasteurellosis. Supernatant preparations containing Lkt produced by M. haemolytica serotype 1, grown in RPMI 1640 medium supplemented with BSA or FBS and without supplements were evaluated during this study. Analysis of obtained Lkt showed presence of 105kDa antigen (SDS-PAGE electrophoresis)...
The most important enzymatic mechanisms which protect an organism against oxidative stress are superoxide dismutase (SOD), peroxidase (Px), e.g. glutathione peroxidase (GSH-Px) and ascorbate peroxidase, catalase and glutathione reductase. Their activity depends on many trace elements. Enzymatic mechanisms, functioning under physiological conditions, prevent the spread of free radical reactions. New...
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