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Molecular probes were generated to study the expression of tyrosine hydroxylase (TH), choline acetyltrans- ferase (ChAT), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP) in the peripheral nervous system of the pig with in situ hybridization. Application of the probes to the sections from the inferior mesenteric ganglion (IMG) in control animals revealed numerous neurons...
Fifteen specimens of the canine mammary gland tumors were examined for the expression of the mRNA c-myc gene by in situ hybridization with the use of a v-myc probe. The expression of c-myc mRNA was observed in fourteen cases of adenocarcinoma. There was no hybridization signal in one case of adenoma. A correlation between the size and the rate of the tumour growth and the hybridization pattern was...
In the present study, the pattern of cyclooxygenase-2 (COX-2) expression in health and inflamed porcine uteri was analyzed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry. On day 3 of the estrous cycle, 50 ml of saline or 50 ml of Escherichia coli (E. coli) suspension containing 10⁹ colony-forming units/ml, were injected into each uterine...
Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of...
Several years ago, the presence of macrophages in the tumor microenvironment was thought to be an inflammatory response to kill the cancer cells. Now, this is clear that the inflammatory cells that exit blood vessels and migrate to the tumor tissue play an important role in cancer progression. Various cells present in the tumor microenvironment enhance cancer growth and invasiveness by secretion of...
Both epidermal growth factor (EGF) and transforming growth factor (TGF) play an important physiological role in the processes of proliferation and differentiation of several different cell types. However, the expression profiles of these factors in domestic bitches endometrium are still poorly recognized. The aim of the present study was to identify and analyze the differential expression of these...
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