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Reliable determination of hookworm nematode parasite species from dogs can be carried out post-mortem by microscopical examination of the buccal cavity of adult worms isolated from the host small intestine. In order to allow a proper diagnosis of the hookworm infection in a living host, we developed a polymerase chain reaction (PCR). Using DNA extracted from hookworm eggs and an oligonucleotide set...
Circulating antigen of Toxocara canis was demonstrated in the sera of experimentally infected rabbits and in patients suspected of the infection. The antigen appeared in the circulation between days 7-14 post infection in animals infected with 5,000 to 20,000 embryonated eggs of the parasite and on 3rd-4th day in rabbits infected with 50,000 eggs. Afterwards, it was present in the sera at irregular...
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