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The aim of the study was to examine the action of green tea polyphenols (GTPP) and their main constituent, epigallocatechin-3-gallate (EGCG), on porcine ovarian granulosa cells. For this purpose, the effect of GTPP and EGCG on cultured porcine ovarian granulosa cell functions, including proliferation, apoptosis, steroidogenesis and response to insulin-like growth factor I (IGF-I) was examined. Proliferation...
The larvae of the black soldier fly, Hermetia illucens, contain high amounts of protein and fat, and so are a good feed source. However, this high fat content hinders the production of such feed. The excess fat must thus be removed to enable the larvae to be used as a feedstock e.g., for aquaculture and livestock. Firstly, the use of hot water treatment as a strategy for extracting fat from dried...
Limited physiological measurements exist for the digestive physiology of porcupines. We measured CH4 emission in three captive Indian crested porcupines (Hystrix indica; 16.1 ± 2.7 kg) fed a diet of pelleted lucerne, and measured feed intake, digestibility, and digesta mean retention time (MRT) of a solute and three particle markers (<2, 10 and 20 mm). Marker excretion patterns suggested secondary...
The objective of the present study was to evaluate the effects of oak acorn (Quercus ilex) intake before and after weaning on lamb growth, meat properties and fatty acid (FA) composition. In total 32 Barbarine male lambs were divided into 4 groups, 8 lambs in each. Before weaning, 16 lambs were reared with their dams on range pasture; each mother-lamb pair received 400 g of barley concentrate. The...
Skeletal muscles are composed of two major muscle fibre types, glycolytic and oxidative, which can be differentiated using their mitochondrial content. Mitochondria are a major generator of reactive oxygen species, and muscles have adapted them to possess oxidative resistance to counteract the oxidative damage. The present study aims to clarify the oxidative tolerance of heat stress (HS) in different...
In the twelve-week study, the effect of feeding olive pulp (OP) without or with Aspergillus awamori on performance, egg quality, serum lipids and antioxidant status of 360 8-week-old laying Japanese quail hens was evaluated. Birds were randomly distributed to 6 groups: control (C) receiving maize-soybean basal diet with no additives, the other supplemented with: 0.1% A. awamori (A) containing 3×106...
Aquaporin-2 (AQP2) is a small, transmembrane protein, active in the principal cells of the renal collecting duct system. Phosphorylation and dephosphorylation of this protein regulate its redistribution in the cell, thereby influencing renal water reabsorption. The aims of the study were to identify and analyse the location of AQP2 phosphorylated at position 261, and to determine its potential role...
The aim of this study was to evaluate the effect of heterotrophically grown docosahexaenoic acid (DHA)-rich thraustochytrid Aurantiochytrium limacinum (AURA) added to dairy cow diet on milk fatty acid profile and milk cheese making properties. The secondary aim was to investigate the effect of pelleting on DHA transfer from diet to milk. Thirty six lactating dairy cows were blocked by parity, number...
This study was aimed to examine the nutritional efficiency of the balanced supply of flavonoids from the crude or ethanol extract of brown propolis on behaviour, productive performance and carcass traits of lambs in feedlot. Twenty four male lambs were divided into 4 groups (6 animals in each) and fed diet supplemented with: crude brown propolis [13 g/kg dry matter (DM)], propolis ethanol extract...
A composition of dietary fat considerably affects the metabolism of nutrients, especially high-fat diet is associated with various metabolic disorders, diabetes and cardiovascular diseases in humans. Studies on rodents are an established model to investigate the pathophysiology of excessive fat in diets, hence the aim of the study was to evaluate the effect of dietary fats different in composition...
Atherosclerosis, a chronic inflammation state of the aorta, is characterised by increased levels of pro-inflammatory cytokines (tumour necrosis factor alpha (TNFα), interleukin (IL)-1β, IL-6). Sheep is used in both cardiovascular and immunological studies; besides, ‘long-day’ ewe can be a model of leptin resistance state. The aim of the study was to examine whether photoperiodic conditions (long-day...
The study aimed to compare in vitro gas and methane production and ruminal fermentation patterns of lucerne hay (Medicago sativa L.), sugar beet pulp (Beta vulgaris L.), maize silage (Zea mays L.), plantago hay (Plantago lanceolata L.), ajuga hay (Ajuga bombycina L.), guelder-rose leaf (Viburnum opulus L.), tomato pomace (Solanum lycopersicum L.), Jerusalem artichoke hay (Helianthus tuberosus) and...
Recently, numerous plant-based preparations have been used for health promotion or disease prevention in animals. Chicory is one of the plants that contain various nutraceutics, mainly inulin type fructans (ITFs) showing prebiotic character. In pigs, a significant proportion of ITFs is fermented in the distal part of the small intestine to lactate and short-chain fatty acids (SCFAs). Previous studies...
Propolis can be an alternative to the use of ionophores in ruminants due to presence of compounds like flavonoids, fenolic acids, esters, fenolic aldehydes and ketones. In this study the impact of solid residue from alcoholic extraction of brown propolis (RBP) added to the diet for feedlot lambs on nutrients intake and digestibility, productive performance, body morphometric measures, carcass characteristics...
The aim of the study was to estimate the genetic parameters of milk fat-to-protein ratio in the first three lactations of Polish Holstein-Friesian cows. Data included 104 875 test-day records of 6299 cows calving from years 2000–2012. Genetic parameters were estimated with a multitrait random regression model using the Bayesian method via Gibbs sampling. The linear model for fat-to-protein ratio and...
To assess the effect of herbal extracts on nutrient utilization, enteric methane emission and growth performance, 15 growing Murrah male buffaloes were divided into 3 groups and offered control diet (total mixed ration; roughage:concentrate ratio 65:35), control diet supplemented with Punica granatum or Tecomella undulata extract at 2% of dry matter intake (DMI) for 90 days. The supplementation of...
In order to explore relationship between the yellow fat and β-carotene metabolism in yaks, contents of β-carotene and retinol in tissues, serum and digestive tract contents were analysed at periods of rich and deficient nutrition. Expressions of β-carotene 15, 15’-monooxygenase 1 (BCO1) gene and protein were also studied using real-time PCR and western blot. It was shown that contents of β-carotene...
The aim of the study was to investigate the effect of the Schizochytrium limacinum marine algae on the fatty acid profile of goat milk, with particular reference to n-3 fatty acids, especially docosahexaenoic acid (DHA) and rumenic acid. Forty dairy goats were randomly allocated to four groups: C – fed with 1500 g alfalfa hay and 600 g concentrate; CMA – received the same forages and concentrate supplemented...
Essential oils are increasingly being used in human health and animal farming as alternatives to antibiotics. The aim of this study was to better understand the mode of antimicrobial action of a natural essential oil mix (EO mix) by comparison with the colistin, as an antibiotic. The growth inhibitory concentration (GIC) of the EO mix and colistin was determined by turbidimetry. Escherichia coli exposed...
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