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Callus induction was achieved from the protonema, whole gametophyte and gametophyte shoot cells in Atrichum undulatum, Bryum caespiticium and Polytrichum commune on modified MS medium containing 4% (w/v) glucose as the carbon source, MS + 4% (w/v) glucose + 1 mg/l 2,4-D + 1 mg/l kinetin, and on MS mineral salts with 10% (v/v) coconut milk. All cultures except cultures containing coconut milk were...
The karyotype structure of Aconitum lasiocarpum (Rchb.) Gáyer was investigated conventionally and by Giemsa C-banding. The chromosome complex of A. lasiocarpum is composed of two distinguishable pairs of long chromosomes and six pairs of considerably shorter chromosomes. Nucleolar organizers are localized on the shorter arms of three (1, 3 and 5) chromosomes. In all analyzed plants the first and third...
Somatic chromosome number was investigated in twenty doubled haploid (DH) lines derived from anther culture of two triticale cultivars. Nine lines have 42 chromosomes, the rest ranging from 39 to 43. The majority of aberrants (90%) are hypoaneuploids (2n = 39 to 41), but within the KR424 line both hypo- (2n = 39 to 41) and hyperaneuploid (2n = 43) chromosome numbers were identified. The observed frequency...
Microspore derivatives occurring in culture of maize microspores were studied by light and electron microscopy. The cultures showed a high degree of heterogeneity throughout the whole period of cultivation. Several types of androgenic structures at different developmental stages were observed, indicating a high level of asynchrony among microspores following the androgenic pathway. During the first...
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