The aim of this work was to develop a simple and fast technique for spatial visualisation of colour cell specific markers in digital microscopy. Colour light microscopy images were transformed into their spatial representation by introducing image brightness as the third dimension. Based on spatial representation, the expression of selected colour specific markers was assessed by their surface, volume, area fraction and colour intensity. This technique was implemented for quantitative analysis of expression of PCNA, Ki67 and bcl-2 in chronic parathyroiditis cases. Comparing to standard, colour sampling techniques, the presented method based on spatial visualisation of the expression of markers in tissue sections, seems an effective and practical application in objective assessment of their distribution in the investigated material.
 R.A. Robb: “Three-Dimensional Visualization in Medicine and Biology”, In: Handbook of Medical Imaging. Processing and Analysis, Academic Press, San Diego, 2000, pp. 685–712.
 E. Nieruchalska, R. Strzelczyk, A. Wozniak, J. Zurawski, E. Kaczmarek and W. Salwa-Zurawska: “A quantitative analysis of the expression of α-smooth muscle action in mesangioproliferative (GnMes) glomerulonephritis”, Folia Morph., Vol. 62, (2003), pp. 451–453.
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Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.