Molecularly imprinting (MI) hydrogels for selective adsorption of trypsin are reported. The trypsin imprinted hydrogels were prepared using a polyethylene glycol (PEG)-based dimethacrylate as a crosslinker and anionic functional monomers. The hydrogel prepared without any functional monomers showed significantly low ability to adsorb a variety of proteins. We optimized the concentration and the length of PEG units of the crosslinkers to achieve the complete removal of the template molecule and suitable selective adsorption. Additionally, the functional monomers chosen were anionic since the template, trypsin, is a basic protein. The adsorption tests for proteins, done on the prepared MI gels, indicated that the MI gel prepared with sodium allyl sulfonate (AS) as a functional monomer showed much higher selective adsorption for trypsin, even though a mixture of trypsin and cytochrome c was used as the protein solution. The selective adsorption was more effective in a NaCl solution in which the non-specific adsorption by a sulfonate is suppressed, similarly to our findings in a previous study. The MI gel prepared with acrylic acid also showed the selectivity, although the adsorption strength was lower than that of the MI gel containing AS. We believe that the present study constitutes the first approach for the selective adsorption of trypsin using PEG-based hydrogels.
 Fischer L., Muller, R., Ekberg, B., Mosbach, K., Direct enatioseparation
of beta-adrenergic blockers using a chiral stationary
phase prepared by molecular imprinting, J. Am. Chem. Soc.,
1991, 113, 9358-60.
 Vlatakis G., Andersson, L.I., Muller, R., Mosbach, K., Drug
assay using antibody mimics by molecular imprinting, Nature,
1993, 361, 645-47.
 Kempe M., Mosbach, K., Direct resolution of naproxen on a
noncovalently molecularly imprinted chiral stationary-phase, J.
Chromatogr. A, 1994, 664, 276-79.
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.