In this paper, the improvement over a novel format for selective solid-phase extraction based on a molecularly imprinted polymer (MIP) is described. A small amount of MIP has been synthesized within the superficial pores of commercial polyethylene (PE) frits and attached to its surface using benzophenone (BP), a photo-initiator capable to start the polymerization from the surface of the support material. Key properties affecting the obtainment of a proper polymeric layer, such as polymerization time and kind of cross-linker were optimized. Prepared composite material was applied to the SPE of TBZ in real samples extracts, showing an impressive clean-up ability. Calibrations showed good linearity in the concentration range of 0.05–5.00 μg g-1, referred to the original solid sample, and the regression coefficients obtained were greater than 0.998. The calculated detection limit was 0.01 μg g-1, low enough to satisfactory analysis of TBZ in real samples. Mean recoveries were about 70 % at different concentration levels with RSDs always ranged below 15% in all the cases.
 Martín-Esteban, A., Sample preparation for chromatographic
analysis, In: Grushka, E., Grinberg, N. (Eds.), Advances in
Chromatography Vol. 51, CRC Press, Taylor & Francis Group,
Boca Raton, Florida, USA, 2014.
 Mayes, A.G., Mosbach, K., Molecularly imprinted polymers:
useful materials for analytical chemistry?, Trends Anal. Chem.,
1997, 16, 321-332.
 Sellergren, B., Martín-Esteban, A., The use of molecularly
imprinted polymers for sampling and sample preparation,
In: Pawliszyn, J., Lord, H.L. (Eds.), Handbook of sample
preparation, John Wiley & Sons, Hodoken, New Jersey, 2010.
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.