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Summary form only given. Pulsed laser fluorescence microscopy (PLFM) using the voltage sensitive dye ANNINE-6 permits measurements of transmembrane potentials of biological cells with a time resolution of five nanoseconds. Several theories have been proposed to explain the change of the membrane conductivity in response to an external electric field, e.g. pore-models, phase transitions due to electro-compression...
We studied the charging of cell membranes in response to ultrashort pulsed high electric fields with a temporal resolution on the same order as the electrical pulse, i.e. nanoseconds. The real-time resolution was achieved by using a pulsed laser (5 ns) as light source, together with a novel voltage-sensitive dye (Annine-6). The laser pulse was synchronized with the pulsed electric field to enable...
Strong pulsed electric fields can induce irreversible pores in the membranes of biological cells and their organelles. Thereby an efflux from the cytoplasm can occur and substances accumulated in the cell become accessible. Therefore pulsed electric fields can be applied for the production of basic materials for chemical, pharmaceutical and food industries as well as for the destruction of micro organisms...
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