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An accurate and convenient assay for mandelate racemase based on polarimetry was developed by making use of the time-dependent decrease of the optical rotation of the substrate (D- or L-mandelate) at 589 nm (Na-D-line) during the enzyme-catalyzed racemization. In comparison to the indirect assay methods hitherto used, this method has the following advantages: (i) it is faster and more accurate than...
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