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Wrinkling of carbon support films is known to limit the resolution of electron microscopy images of protein two-dimensional crystals. The origin of carbon wrinkling during preparation of the support films was investigated by reflected light microscopy. We observed that carbon films go through several states during their preparation. While dried carbon films have a tendency to be wrinkled, a flat state...
We have recently shown that carbon films used as specimen support films in electron microscopy were highly wrinkled (Schmutz et al., J. Struct. Biol. 112 (1994) 252). We report here on a further analysis of the planarity of carbon films at the different steps of their preparation, by reflection light microscopy. Carbon films evaporated on mica and floated on a water surface presented straight striations...
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