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Endocrine cells produce large amounts of one or more peptides. The post-translational control of selective production of a single protein is often unknown. We used 3 unrelated approaches to diminish PKCε in rat islets to evaluate its role in preferential glucose-mediated insulin production. Transfection with siRNA (siR-PKCε) or expression of inactive PKCε (PKCε-KD) resulted in a significant reduction...
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