The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Binding isotope effects are a sensitive measure of changes in molecular vibrational character that occur during ligand- receptor binding. In this study, we have measured isotope effects on the binding of glucose to human brain hexokinase using the ultrafiltration method, with the following results: 0.991š0.001, 0.908š0.003, 1.010š0.001, 0.974š0.002, 1.022š0.002 for [14C]-glucose mixed with [1-3H]-,...
Pertussis toxin catalyses the transfer of the ADP ribosyl moiety from NAD + to a specific cysteine residue of several G-proteins, four amino acids from the C-terminus. In absence of an acceptor protein, pertussis toxin acts as NAD + glycohydrolase. Primary and secondary kinetic isotope effects were determined for the hydrolysis reaction, using 3 H, 14 C and ...
Ricin A-chain catalyses the depurination (N-ribohydrolysis) of adenosine at position 4324 of mammalian 28s rRNA to destroy the binding site of eukaryotic elongatial factor 2. One ricin molecule is sufficient to depurinate all ribosomes in a single cell, making ricin one of the most potent biological toxins. Enzymatic depurination reactions for AMP and inosine are catalysed by AMP nucleosidase and...
Ricin A-chain (RTA) is a potent toxic protein which inactivates ribosomes by catalysing the depurination of adenosine 4324 in 28s rRNA. The minimal substrate for recognition by ricin A-chain has been reported to be a RNA 10-mer featuring a stem-loop structure containing the GAGA tetraloop. The mechanism of action of RTA is similar to N-nucleoside hydrolases which have oxycarbonium character at the...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.