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Indium tin oxide (ITO) surfaces were modified with ssDNA by coupling oligonucleotides to a monolayer of 12-phosphonododecanoic acid (12-PDA) on ITO surfaces. This coupling involved the formation of an amide bond between the carboxylic acid moiety of 12-PDA to the amine group of a 5′-aminopropyl-labeled single strand of DNA. The self-assembled monolayer of 12-PDA and surface-attached oligonucleotides...
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