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Parathyroid hormone (PTH) provokes cell division of osteoblasts in vitro and is anabolic in vivo when administered intermittently. We studied the effect on bone formation in vitro by rat primary calvarial osteoblasts of the short-term addition of rat PTH(1-34) (10 -12 , 10 -9 , and 10 -7 mol/L) to the medium. Our aim was to determine whether intermittent...
Adenosine triphosphate (ATP) and other purinoceptor agonists cause a transient rise in [Ca 2+ ] i in cultured osteoblastlike cells and have a mitogenic effect, as does parathyroid hormone (PTH), and there is evidence that ATP and PTH can act synergistically on osteoblasts. The likelihood that nucleotides, acting through purinoceptors, are important local factors in bone remodeling...
We have investigated the influence of substrate topography on the timing and location of bone formation by rat osteoblasts. 250 μm thick slabs of dental tissues were used intact or had a rectangular grid of grooves ( 350 μm wide and of variable depth) cut with a diamond wheel. They were then seeded with rat calvarial osteoblasts and cultured in MEM with 10% FCS at 37°C in 5% CO 2 . Ascorbic...
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