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To examine the effect of microglial conditioning medium (MCM) on damaged neurons, cultured neurons exposed to glutamate were incubated with MCM. More neurons survived in these groups, particularly in the case of glutamate stimulation of microglia. Therefore cultured neurons exposed to glutamate were incubated with MCM at predetermined intervals after glutamate stimulation. Neuronal cultures using...
We investigated expression of macrophage inflammatory protein-1 (MIP-1) α and β in human astrocytoma cell lines and surgical specimens of astrocytic tumors. Enzyme-linked immunosorbent assay (ELISA) showed constitutive secretion of MIP-1α protein in only one and MIP-1β in none of 7 cell lines tested. However, MIP-1α production was increased in three cell lines by stimulation with lipopolysaccharide...
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