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A 3D imaging system using a double-axis fluorescence microscope provides a practical way to reconstruct 3D images of micro-objects from images simultaneously acquired from the two orthogonal directions. In this paper, we show experimental results of 3D image reconstruction using a water plant specimen as an object.
We have developed a double-axis microscope, in which two microscopic imaging systems are orthogonally placed, for reconstructing 3D images of micro specimens. We first show the optical properties of the microscope by analyzing 3D observation images of a small fluorescent sphere. We next propose a position adjustment method for 3D images taken through the microscope using the small sphere as an optical...
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