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Deamidation of Asn residues is one of the major chemical pathways of degradation of proteins and peptides. Adrenocorticotropic hormone (ACTH), a 39-amino acid polypeptide with a single Asn residue, was shown in this study to be a useful model polypeptide for the study of the effects of pH and buffer concentration on the rate and pathway of deamidation. The disappearance of ACTH and appearance of deamidated...
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