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Fluorescent ethenodeoxyadenosine (dA') or/and ethenodeoxycytidine (dC') were site-specifically inserted into four oligonucleotides through phosphoramidite chemistry on an automated DNA synthesizer, their nucleoside compositions were confirmed by enzymatic digestion analysis. The UV melting behaviors indicate that the conformation of modified duplex is not affected when incorporated into modified nucleosides...
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