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The acetylation of ubiquitin by [acetyl-1- 13 C]aspirin has been studied using 2D NMR methods. Studies performed in a 50:50 H 2 O:D 2 O medium show doubling of the acetyl carbonyl resonances, indicating that all of the stable adducts formed involved amide linkages. Assignment of the heteronuclear multiple quantum coherence (HMQC) resonances was accomplished based on comparison...
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