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A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (n=39) and patients with other neurodegenerative disorders (n=38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40ng/mL in CSF and plasma/serum, respectively. We identified...
The development of an MRM assay for the measurements of six inflammatory markers is presented. We report a robust and sensitive quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing. The assay has a dynamic range with 4 orders of magnitude and the LOQs are in the attomolar range. We used plasma from Huntington's disease gene carriers and healthy...
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