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Since the 1950’s nutrition recommendations have focussed on the replacement of saturated fats in the diet with polyunsaturated fats, a strategy that continues to this day. Despite supporting evidence from clinical trials for the advantages of Mediterranean diets, there has been less attention paid to the role of monounsaturated fats. It has been known for many years that diets high in linoleic acid...
Omega–3 and omega–6 polyunsaturated free fatty acids (PUFA–FFA) are precursors to potent downstream lipid mediators that are regulators of inflammation. We describe the development and validation of a novel and sensitive method for quantification of individual PUFA–FFA in a dried blood spot using liquid chromatography tandem mass spectrometry (LC–MS/MS). Lipids were extracted from dried blood spot...
Randomised controlled trials are the ideal way to assess the effects of interventions. Small trials are useful for generating pilot data to determine samples sizes for larger trials, but can produce unreliable or biased results if they are considered in their own right. We investigate the impact of small sample sizes due to either inadequate recruitment targets or high attrition rates on the results...
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