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The death of yeast treated with hydrogen peroxide (H 2 O 2 ) shares a number of morphological and biochemical features with mammalian apoptosis. In this study, we report that the permeability of yeast nuclear envelopes (NE) increased during H 2 O 2 -induced cell death. Similar phenomena have been observed during apoptosis in mammalian tissue culture cells. Increased...
The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that...
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