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We report the development of a microfluidic device that is the first of its kind to generate uniform arrays of individually addressable cell seeded calcium alginate gels for 3D cell culture using electrowetting on dielectric (EWOD) digital microfluidics (DMF). This is combined with EWOD DMF's multiplexing abilities to demonstrate how a single device is capable of forming cell seeded alginate hydrogels,...
Metallothionein (MT) isoforms have not been studied in papillary thyroid cancer. We examined how the functional MT1 and MT2 isoforms were expressed in papillary thyroid cancer (KAT5) cells. We demonstrated that KAT5 cells expressed eight functional MT1 and MT2 isoforms induced by cadmium. Elevated calcium and activated ERK1/2 predated MT expression. The inhibition of either calcium or ERK1/2 significantly...
Metallothionein (MT) expression in carcinogenesis of thyrocytes is unknown. We demonstrated that cadmium induced transcription of all functional MT-1 and MT-2 isoforms and promoted the cell cycle from the G1 to the S phase in thyroid cancer cells, which can be suppressed by the ERK inhibitor. Cadmium exposure stimulated intracellular calcium and the phosphorylation of ERK1/2. Therefore, a common pathway...
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