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We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22kDa (22K) and 20kDa (20K). An oriented-antibody sensor surface specific for both hormone isoforms was assembled by using the biotin–streptavidin system. The immunosensor functionality was checked for the direct detection of the 22K hGH isoform...
A surface plasmon resonance immunoassay has been developed to determine human growth hormone (hGH) directly and without pre-treatment in human serum samples. A binding inhibition immunoassay was employed. Antibody concentration, assay buffer and regeneration solution have been optimized in order to reach the best performance and the lower non-specific binding of the matrix components to the sensor...
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