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Intrazellulärer Transport Eine Methode für den Transport von nativen Proteinen in das Cytosol lebender Säugerzellen mit sofortiger Bioverfügbarkeit beschreiben L. Li, S. Q. Yao et al. in ihrer Zuschrift auf S. 1548. Die Autoren berichten über neuartige Konjugationsreaktionen, die die kovalente Modifizierung von nativen Proteinen mit zellgängigen Polydisulfiden (CPDs) ermöglichen. Das Bild zeigt ein...
Intracellular delivery of therapeutic proteins is highly challenging and in most cases requires chemical or genetic modifications. Herein, two complementary approaches for endocytosis‐independent delivery of proteins to live mammalian cells are reported. By using either a “glycan” tag naturally derived from glycosylated proteins or a “traceless” tag that could reversibly label native lysines on non‐glycosylated...
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