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This study was conducted to investigate the influence of recipient cytoplasm on the development of somatic cell nuclear transfer (SCNT) embryos, using recipient oocytes and donor cells that were obtained from cats, cows, and pigs. Bovine and porcine oocytes were collected from ovaries obtained at a slaughterhouse, and cat oocytes were collected from ovaries obtained at local veterinary clinics following...
This study was conducted to examine the effect of the donor cat's reproductive cycle stage on in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro development of oocytes recovered from ovaries that were collected and stored at 35 o C for a short period (1-6h). Based on the presence or absence of follicles and corpora lutea, the ovarian pairs collected were classified into...
Bovine oocytes (90 to 99 µm in diameter) were isolated from early antral follicles (0.5 to 0.7 mm in diameter). Cumulus-oocyte complexes (COC) with pieces of parietal granulosa were embedded in collagen gels and cultured for 14 d. After in vitro growth culture, oocytes recoveredfrom the collagen gels were further matured, fertilized and cultured in vitro, and then were transferred to recipient cows...
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