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Background: Previously, we showed that human peripheral blood mononuclear cells (PBMCs) that were cryopreserved, stored for 14 months at: (1) −150°C; (2) −150°C except temperature cycled for 104h; and (3) −80°C and then thawed had numerous genes that were differentially regulated compared to freshly isolated cells. Study Design and Methods: We have expanded our initial report to include a 6.5 year...
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