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Lactase phlorizin hydrolase (LPH; EC 3.2.1.62) is a membrane-bound, family 1 β-glycosidase found on the brush border of the mammalian small intestine. LPH, purified from sheep small intestine, was capable of hydrolysing a range of flavonol and isoflavone glycosides. The catalytic efficiency (k cat /K m ) for the hydrolysis of quercetin-4'-glucoside, quercetin-3-glucoside,...
The conformation of 4-thiocellobiose bound to β-glucosidase from Streptomyces sp. has been studied by 1 H-NMR transferred nuclear Overhauser effect spectroscopy (TR-NOE). Thiocellobiose behaves as an inhibitor of this glucosidase when cellobiose is used as substrate. NOE measurements and molecular mechanics calculations have also been performed to estimate the probability distribution of conformers...
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