Sheila MacIntyre

chapter

The Fish Pathogen Yersinia ruckeri Possesses a TTS System

Deephti K. Gunasena, Jenny R. Komrower, Sheila Macintyre

Advances in Experimental Medicine and Biology > The Genus Yersinia > Pathogenesis and Host Interactions > 105-107

chapter

Mutagenesis Elucidates The Assembly Pathway and Structure of Yersinia pestis F1 Polymer

Joanne E. Kersley, Anton V. Zavialov, Elham Moslehi, Stefan D. Knight, more

Advances in Experimental Medicine and Biology > The Genus Yersinia > Pathogenesis and Host Interactions > 113-116

article

Allosteric Mechanism Controls Traffic in the Chaperone/Usher Pathway

Xiao Di Yu, Anatoly Dubnovitsky, Alex F. Pudney, Sheila MacIntyre, more

Structure > 2012 > 20 > 11 > 1861-1871

Many virulence organelles of Gram-negative bacterial pathogens are assembled via the chaperone/usher pathway. The chaperone transports organelle subunits across the periplasm to the outer membrane usher, where they are released and incorporated into growing fibers. Here, we elucidate the mechanism of the usher-targeting step in assembly of the Yersinia pestis F1 capsule at the atomic level. The usher...

article

Large Is Fast, Small Is Tight: Determinants of Speed and Affinity in Subunit Capture by a Periplasmic Chaperone

Xiao Di Yu, Laura J. Fooks, Elham Moslehi-Mohebi, Vladimir M. Tischenko, more

Journal of Molecular Biology > 2012 > 417 > 4 > 294-308

The chaperone/usher pathway assembles surface virulence organelles of Gram-negative bacteria, consisting of fibers of linearly polymerized protein subunits. Fiber subunits are connected through ‘donor strand complementation’: each subunit completes the immunoglobulin (Ig)-like fold of the neighboring subunit by donating the seventh β-strand in trans. Whereas the folding of Ig domains is a fast first-order...

article

Conserved Hydrophobic Clusters on the Surface of the Caf1A Usher C-Terminal Domain Are Important for F1 Antigen Assembly

Anatoly P. Dubnovitsky, Zoe Duck, Joanne E. Kersley, Torleif Härd, more

Journal of Molecular Biology > 2010 > 403 > 2 > 243-259

The outer membrane usher protein Caf1A of the plague pathogen Yersinia pestis is responsible for the assembly of a major surface antigen, the F1 capsule. The F1 capsule is mainly formed by thin linear polymers of Caf1 (capsular antigen fraction 1) protein subunits. The Caf1A usher promotes polymerization of subunits and secretion of growing polymers to the cell surface. The usher monomer (811 aa,...

article

Structure and Biogenesis of the Capsular F1 Antigen from Yersinia pestis - Preserved Folding Energy Drives Fiber Formation

Anton V. Zavialov, Jenny Berglund, Alexander F. Pudney, Laura J. Fooks, more

Cell > 2003 > 113 > 5 > 587-596

Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding...

article

Structural and Functional Similarity between Yersinia pestis Capsular Protein Caf1 and Human Interleukin-1β ^†

Vyacheslav M. Abramov, Anatoly M. Vasiliev, Raisa N. Vasilenko, Nataly L. Kulikova, more

Biochemistry > 2001 > 40 > 20 > 6076 - 6084

article

Study of the intergenic exeF-exeG region and its application as a simple preliminary test for Aeromonas spp.

Andrey V. Karlyshev, Sheila MacIntyre

FEMS Microbiology Letters > 1996 > 137 > 1 > 37-44

The exeF-exeG intergenic regions from different hybridization groups (HG) of Aeromonas were studied by PCR amplification using a single pair of primers. Six main classes of PCR products were identified according to size: 360 bp, 320 bp, 280 bp, 230-240 bp, 220 bp and 160 bp. Direct sequencing of the PCR products indicated that the shorter intergenic regions had probably originated from deletion...

article

Specific high affinity binding of human interleukin 1β by Caf1A usher protein of Yersinia pestis

Vladimir P. Zav'yalov, Tatiana V. Chernovskaya, Elena V. Navolotskaya, Andrey V. Karlyshev, more

FEBS Letters > 1995 > 371 > 1 > 65-68

Understanding the interaction of Yersinia pestis with the key components of the immune system is important for elucidation of the pathogenesis of bubonic plague, one of the most severe and acute bacterial diseases. Here we report the specific, high affinity binding (K _d = 1.40 10 ^- ¹ ⁰ M ± 0.14 10 ^- ¹ ⁰ ) of radiolabelled human interleukin 1β (hIL-1β)...

article

Cloning and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida

Andrey V. Karlyshev, Sheila MacIntyre

Gene > 1995 > 158 > 1 > 77-82

The Aeromonas salmonicida (As) exe gene cluster, an additional member of the pul-related operon family required for general signal-sequence-dependent secretion of proteins from Gram ^[ ^m ⁱ ⁿ ^u ^s ^] bacteria, was cloned in the broad-host-range cosmid pLAFR3. Twelve genes, exeC-N, were identified by partial nucleotide (nt) sequence analyses (exeE-N) or determination...

article

Topological analysis of the amino-terminal region of lactose permease using the Escherichia coli outer membrane protein, OmpA, as a marker

Sheila MacIntyre, Marie-Luise Eschbach, Heinz Schwarz, Ruth Ehring

FEBS Letters > 1989 > 247 > 2 > 396-400

LacY-ompA fusions, encoding the N-terminal 50, 71 or 143 residues of lactose permease, were constructed. The observed orientation of the OmpA part of each hybrid protein with respect to the plasma membrane supports current models of the N-terminus of Lac permease. Hybrids possessing the entire mature OmpA were very stable; those with only a part thereof were much less stable. Due to their in vivo...

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Search results for: Sheila MacIntyre

The Fish Pathogen Yersinia ruckeri Possesses a TTS System

Mutagenesis Elucidates The Assembly Pathway and Structure of Yersinia pestis F1 Polymer

Allosteric Mechanism Controls Traffic in the Chaperone/Usher Pathway

Large Is Fast, Small Is Tight: Determinants of Speed and Affinity in Subunit Capture by a Periplasmic Chaperone

Conserved Hydrophobic Clusters on the Surface of the Caf1A Usher C-Terminal Domain Are Important for F1 Antigen Assembly

Structure and Biogenesis of the Capsular F1 Antigen from Yersinia pestis - Preserved Folding Energy Drives Fiber Formation

Structural and Functional Similarity between Yersinia pestis Capsular Protein Caf1 and Human Interleukin-1β ^†

Study of the intergenic exeF-exeG region and its application as a simple preliminary test for Aeromonas spp.

Specific high affinity binding of human interleukin 1β by Caf1A usher protein of Yersinia pestis

Cloning and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida

Topological analysis of the amino-terminal region of lactose permease using the Escherichia coli outer membrane protein, OmpA, as a marker

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