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For efficient coupling of droplet‐based microfluidics with mass spectrometry (MS), a spyhole drilled on the top of a microchip is used to sample the passing droplets by electrostatic‐spray ionization (ESTASI) MS. The technique involves placing an electrode below the chip under the spyhole and applying high‐voltage pulses. Electrospray occurs directly from the spyhole, and the droplet content is analyzed...
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