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Aims: A simple and rapid method (designated thermolysis) for extracting genomic DNA from bulk fungal strains was described.
Methods and Results: In the thermolysis method, a few mycelia or yeast cells were first rinsed with pure water to remove potential PCR inhibitors and then incubated in a lysis buffer at 85°C to break down cell walls and membranes. This method was used to extract genomic DNA...
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