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To explore the effects of Mucins (MUC)1‐shRNA on the proliferation and hypoxia inducible factor (HIF)‐1alpha expression of human cholangiocarcinoma (CCA) QBC939 cells in vitro. MUC1‐shRNA was constructed and transfected with Lipofectamine™ 2000 into cultured CCA cells. MUC1 mRNA and protein expression levels were determined by RT‐PCR and Western blot, respectively. The cellular proliferation and HIF‐1alpha...
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